Expression Systems

Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.

Expression Systems: Methods Expressuniquely fills this need. It covers expression across a broad range of systems, including the following:

CHO cells
Lactococcus lactis and other gram positive bacteria
S. cerevisiae
Transfected insect cells
Lentiviral vectors

The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.