Bioseparations Engineering: Principles Practice and Economics

Multidisciplinary resource for graduate studies and the biotechnology industry Knowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts and new bio products from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes and heart disease or products for industry and agriculture, the science and technology of bio separations are the keys to delivering these products in a purified form suitable for use by people. The methods, theory and materials that reduce the science of bio separations to practice, whether in the laboratory or the plant, are the subjects of Bio separations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for: * Isocratic and gradient chromatography * Sedimentation, centrifugation and filtration * Membrane systems * Precipitation and crystallization Topics addressed within this framework are: stationary phase selection; separations development; modelling of ion exchange, size exclusion, reversed phase, hydrophobic interaction and affinity chromatography; the impact of regulatory issues on chromatography process design; ....