Applied Molecular Genetics

This book is a concise, easy to read guide to the scientific foundations of the most commonly used applications of molecular genetics in biological and biomedical research. For students and researchers alike, this book fills the conceptual gap between published protocols and reports of experimental results by explaining how various molecular techniques are combined to achieve research success. Full chapters are devoted to five major areas of applied molecular genetics, manipulation of genomic DNA, isolation and characterization of gene coding sequences, the polymerase chain reaction, gene expression in cultured cell systems and construction of transgenic multicellular organisms.